BIOLOGY 1AL Lecture Notes - Lecture 8: Molar Attenuation Coefficient, Daniel E. Koshland Jr., Numerical Aperture
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10 fold dilution from 1 m to 0. 1m or 100mm to 1000mm. You have 1m of nacl; you want to make 100 mm of nacl. Take 1ml and add it to 9ml of the solvent (water) If you want to make a 1mm, you repeat this twice. Light source monochromator sample solution digital display meter. Take solvent (water) and put it in solution and zero it to make sure water has no absorbance at all. A = -log (t), percent transmission = t = i t /i o. Absorbance = c l z = [concentration] x (length) x (molar extinction coefficient. Linear relationships between absorbance of a molecule and concentration in solution. Closing the aperture diaphragm can change contrast to improve image. Resolution: ability to distinguish 2 points; defined by the distance between the 2 points. Resolution = (0. 61 x lambda)/(numerical aperture of objective lens)
