ESPM 131 Lecture Notes - Lecture 30: Biome, Ribosomal Rna
1 May 2018
School
Course
Professor
Lecture
30
:Ecological Principles I4/13
.
Diversity :soil is the most diverse microbial ecosystems
-
biodiversity :deg of var of life Forms wl in sp,ewsys,
biome
,planet
-
species :standard concept doesn't really apply to Microbes
;
grp capable of interbreeding
org
's genetic material comes from 1or 2parents that an related ←hone gene trane.Kt)
.transformation :DNA uptake from env
.thnsdrtion :
DNA from rinses
.
conjugation :transfer btw but
-metrics :
.d- diversity :wlin sample
-richness :ONS (taxon richest sum of richness (phylog .richness
)
↳operational taxonomic units
-
evenness :how types are distributed
indices like Shaman
,
Simpson ,
ek
-how to dessifn ?
.
morphology
.OTVS :taxon level of sampling selected by user to be used in ashdy ,such as indiv
,
pop,sp ,genera ,
or strains → an arbitrary measure to define abiological unit
ie .but w />971 .
165 sin
.
form an OTU (aka phylo types)
↳for species
.
now approaches :w/ whole genome sequence
-GANI :
genome
.wide avg .nucleotide ID (species =96.51 .)
-but whole genome can be ~851. even if NOT. RRNA
-recent transfers won't be different !
.defining ON :
.
9. -dmwtd
-align sq .by arow
orys
-calc .Sin Hist
-
gwpsq .Ydefined dist off
,h€#_
count nof groups (dtiv )
-count e compare across samples nseq . wlin groups Atdid #dots in bikes 'evenness
-VCWST:anlnid .based
,Md .
-high -ID clustering ,
#of bubbles offtf ONS =richness of sample
Document Summary
4/13 is the most diverse microbial ecosystems of var of life forms wl in sp , ewsys biome. : standard concept doesn"t really apply to microbes grp capable of interbreeding. "s material comes from 1 genetic org transformation : dna uptake from env or 2 parents that an related hone trane. Kt) gene thnsdrtion :dna from rinses conjugation : transfer btw but evenness. Ons (taxon richest indices like shaman sum of richness (phylog . : how types are distributed d- diversity : wlin sample taxon level of sampling selected by user. ,h #_ avg . nucleotide id ( species = 96. 51 . ) by a off. But whole transfers won"t be different ! a shdy , to define a approaches : w/ whole defining on : to be used in. For species sp pop , ie . but if not . ~ 851 . even indiv unit w /
