MCELLBI 104 Lecture Notes - Lecture 30: Outsideinside, Karyopherin, Cell Membrane
8 Apr 2016
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Slide 2: eukaryotic cells, all sorts of different membrane-bound compartments. Slide 4: transcription, gene regulation, etc, er: lipid synthesis, also protein synthesis, secrete proteins to outside world. 8 amino acid residues that are recognized by nuclear import machinery. Will take protein and through nuclear pore and into cell: signal sequence: small stretch of aa, main purpose is to work as a zip code, tell cellular machinery where protein should be distributed to. Slide 11: ran is key regulator of process, encode small gtpase. Slide 12: gef and gap, even when nuclear breakdown happens and finding kinetochores, Mt know where it is b/c ran-gtp is high and ran-gdp is low: concentration gradient to show where chromosomes are (where nucleus used to be, where ran-gtp is high probably) Slide 14: fine tubular network, electron microscopy allowed us to distinguish b/w two types, smooth and rough, based on appearance, rough er: studded with all these dots that are ribosomes.
