NUSCTX 103 Lecture Notes - Lecture 7: Glucokinase Regulatory Protein, Glucokinase, Phosphofructokinase

• School: Berkeley
• Department: Nutritional Sciences And Toxicology
• Course: NUSCTX 103
• Professor: Olzmann

Document Summary

Lecture b (professor sul): handling of plasma glucose and glycolysis. Study guide: what three enzyme-catalyzed steps regulate the catabolism of glucose to pyruvate, 1glucose + atp (hexokinase/m hexokinase i/l glucokinase) -->1 glucose-6-phosphate. + adp: use 1 atp (mg2+, hexokinase i (muscle // low km- for low gluc conditions/scavenge, against gradient ) coordinates w /glut 4 (low km) i) ii) Inhibited by: g lucose-6-phosphate [pdt: glucokinase (liver // high km- for high gluc /respond even at high conc ) coordinates w/ glut 2 (high km) i) ii) Activated by: fructose-1-phosphate* (binds to gkrp) *in high fruc cond. Inhibited by: f ructose-6-phosphate (binds to gkrp) (1) see paper sent to cytosol (active) (1) kept in nucleus (inactive) 3) 1 fructose-6-phosphate + atp (pfk-1) --> 1 fructose-1,6-bisphosphate -26 kj/mol: use 1 atp (mg2+, activated by : amp (allosteric) & fructose-2,6-bisphosphate (high gluc/inc insulin) c) 10) 2 phosphoenolpyruvate + 2 adp (pyruvate kinase) --> 2 pyruvate + 2 atp.