PLANTBI C112 Lecture Notes - Lecture 7: Plasmid, Reverse Genetics, Ribosomal Rna
19 Sep 2018
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On knock out the doom - copy using targeted disruption gene: when the plasmid gene is the only copy , turn it. Off by removing the inducer: wait for the existing protein to turn over robs . the cells to see what happens in the absence of the essential gene i protein. Changing gene regulation w/ an inducible promotor some proteins are needed the in all only under specific conditions. 3) ie promoter not active you t substrate for rxn needing protein r sing these inducible promoters we can insert them in front of the make random mutations sent screen forward genetics reverse genetics. Microbial genomics con figs output - of comp . algorithms, strings of connected seq in reads closed by gaps attempting. Pk prod sequenced to determine intervening seg . btrconh. gs.
