BIOL 311 Lecture 2: Note (2)
11 Mar 2018
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Group of processes used to manipulate and measure nucleotide sequences. Terms: genomics molecular characterize of genomes, power: Selective expression of a gene of interest. Knockouts cells/animals with a deleted gene or one dramatically decreased. Makes permanent change in the dna sequence: ex: changes c-g to a-t, types of mutation. Frameshift reading frame change: mutations w/ yeast cells: Grow at one temp, but not another. Non-permissive temp won"t grow 36 c. Permissive temp will grow 23 c: complementation analysis. Determine if mutations are on the same gene. Will not complement if they are on the same gene: mutation types. Redundant pathways: general concepts and principles, discovery of restriction nucleases, 1) 1970"s. Before: could not manipulate dna (could purify it) After: could: 2) restriction nucleases can cut dna, 3) restriction nucleases discovered in bacteria help ward off viruses, 4) now are commercially available. Can cut with blunt ends (not preferred)
