BIOL 311 Lecture Notes - Lecture 11: 2-Mercaptoethanol, Electron Transport Chain, Aldehyde
19 Aug 2016
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In the former case a single cell is injected such as nuclear transfer from a somatic (non-germ) cell to an egg (see class ppt). Another example is the injection of a single neuron with a fluorescent dye so that one can trace its synaptic connections in a multicellular tissue such as brain or ganglion. In some cases more than one answer is possible. Substantiate your choice(s): determining which proteins are selectively synthesized in cells in response to. Epidermal growth factor-answer: gel electrophoresis preferably with 35s-methionine labeling: determining how much of a particular protein is present in cells. Seldi-tof: determining differences in mrna expression using cdna technology between two adjacent cells within a tissue. In this case you would use laser capture microscopy. In situ hybridization would work as well, but the question calls for cdna microarray technology: challenge: you purify a protein of interest using native gel electrophoresis and find that its relative molecular weight is 200,000 daltons (200 kd).
