CAS BI 216 Lecture Notes - Lecture 12: Dna Glycosylase, Ap Site, Ap Endonuclease
19 May 2017
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Lecture 12 3/23/17 (chapter 7 and chapter 8) Oxidation damage to dna: many pathways - o2 -> converted to reactive oxygen species (ros, hydroxyl radical, >100 kinds of dna damage from ros, quantitatively - 8-oxo-guanine is most prevalent, 8-oxo-guanine pairs frequently with adenine, go mutagenic pathway. Nucleotide excision repair: bi203/bi213 repairs uv light damage and large forms of chemical damage. Transcription: promoter - where rnap binds and starts mrna synthesis, also rrna and trna, consensus sequences recognized by rnap (focus on bacteria) 35 sequence -> ttgaca: about 17 bp in between, tss (transcription start site) ( at -1/+1, everything to the right is downstream - direction it is sequenced, everything to the left if upstream. Ttgaca tataat (-3) (-2) (-1) tss (+1) (+2) (+3) 5" - gauc - 3: transcribed strand. 3" - ctag - 5: sequence mrna is similar to the non-transcribed strand, mrna.
