CAS BI 203 Lecture Notes - Lecture 8: Southern Blot, Autoradiograph, Epitope
18 Jan 2020
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Howlong1cycles does it take to get to 509o. 2 southern blotting dna good for detecting large changes in genomic dna probe longersegment of dna 400 500 bp to generate morespecific hybridization. 2 separation by formaldehyde containing agarose get electrophoresis. 4 in situ hybridization used to detect homologous dna or rnasequences in cell extracts intact cells chromosomes and. G antibodies as probes to visualize proteins studyinggene or proteinfunction often requires visualization of a particularprotein. Antibodies proteinsproduced by the immune system typically by blymphocytes. Faced w antigen immune system uses trial an error to make a lot of antibodies to find correctantibody bing. ie. E variableregion constantregion antibodies bind to foreignsubstances antigens which targets them for attacks by immune system cells. Immune system can producemillions of differentantibodies that can bind to different antigens. Antibodies that bind a desiredantigen can begenerated by inoculating an animal with that antigen oncepurified antibodies can beused to identify a protein of interest common technologies l.
