CAS BI 203 Lecture Notes - Lecture 1: Disulfide, Ribonuclease, Thiol
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Elevator to 5th floor glass door to left that"s locked dial 8-5176 on the phone . Sept 22, oct (look @ syllabus) , dec 1st quizzes. Reading notes: reductive cleavage of disulfide bridges in. Total cleavage of the 4 disulfide bridges in ribonuclease (rnase) may be achieved w. complete loss of enzyme activity. Armour was reacted w. thioglycollic acid in 8m of urea (ph 8. 5) for. The reduced ribonuclease was precipitated w. acetone and 1m hcl and washed 3 times w. acetone and twice w. cold ether. An alt method was used for following the extent of reduction involved selective carboxymethylation of the sulfhydryl groups of reduced ribonuclease by rxn under nitrogen with iodacetic acid for 2 hrs. Doing the reductions in the 8m of urea for shorter periods of time or at slightly lower ph values resulted in partially reduced materials w. varying degrees of activity.
