CAS BI 410 Lecture Notes - Lecture 29: Pdx1, Catenin, Contact Inhibition
22 May 2017
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Cxcr4 receptor dncxcr4; protrusions stained receptor there is no directed migration; And what is with sdf1 gof without sdf1 there are outer cell protrusions the role of sdf1 less protrusions. A-d: tracked interactions affect efficiency of movement of cells single or in cluster. migration: run vs. E-h: chemotaxis assays of control cells and dncxcr4 cells. Then mixed together. efficiency improves as cell density increases, but reaggregation does essential for neural crest cell sdf1-directed chemotaxis. Original arrangement of cells is not matter not required. A-d: cell cooperation is allows for efficient collective chemotaxis. A-d: single cells run with a greater speed, but spend most of the time tumbling. Cluster cells run and tumble but do both with higher average speed. Protrusions are formed and collapsed in cluster tumbling cells. E-h: dncxcr4 cells do not migrate (mostly tumble). But when mixed, are able to migrate toward signal. autonomous behavior.
