BIOMG 1350 Lecture Notes - Lecture 2: Cell Membrane, Immunofluorescence, Eyepiece
2 Sep 2016
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Light microscopes are still most commonly used but has limitations: many cells appear transparent and are hard to see. Dyes can be used to reveal cell structures. Chemical properties of dyes allow them to color particular cell components differently. Dyes are usually not very specific or sensitive. Sensitivity: ability to detect small amounts of something. Specificity: antibodies or gfp fusion proteins can be used. Reflects light below a certain wavelength and transmits light above a certain wavelength. The light will pass through objective lens and the object. The object takes in the light at a blue color wavelength and emits it at a green color wavelength. The light will pass through the beam splitting mirror instead of reflective off it. The light passes through the emission filter and into the eyepiece. Antibody specificity: an individual animal can make billions of different antibody molecules, each with distinct antigen binding sites. Each antibody recognizes its antigen with great specificity.
