MCDB 2150 Lecture 5: Class 5 Translation
8 Feb 2017
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Document Summary
Translation starts at the start codon: aug. Translation stops at one of the three stop codons: uag, uaa, and uga: no trna corresponds to these codons so the chain of amino acids is released from the ribosome. In the 1960s, scientists knew dna was comprised of atcg, but didn"t know how many nucleotides of rna encoded each amino acid. They knew proteins were made of amino acids and that there were 20 distinct amino acids. Determining the length of each codon: francis crick and colleagues determined that each codon but me three nucleotides by deleting or inserting one nucleotide to a strand of a known length. This also demonstrated that the code was non- overlapping. Determining the identity of each codon: synthesize rna of different known nucleotide sequences and wee which peptide sequences are generated. Reading frames: the start codon used by the ribosome during translation determines initiation of translation and the reading frame .
