MCDB 2150 Lecture 18: Class 18 Biotechnology- Gene Editing and Screening Libraries
10 Mar 2017
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Class 18 biotechnology: gene editing and screening libraries. Video: crispr, the crispr/cas system serves as a part of bacteria"s immune system. Lecture: crispr, the crispr/cas system serves as a part of bacteria"s immune system. 5 nucleotide sequence) just downstream of targeted cut site: this is required for. Screening a library - a library is a collection. Cas9 protein plasmid-crispr library: each virus carries coding sequence of cas9 protein and coding sequence of a single (unique) crrna. Infect cells with virus and optionally give the cells a treatment (ex. Isolate genomic dna: some fragments will contain one or more genes, a few fragments will contain your gene of interest, ligate all genomic dna fragments into cloning vectors cut with the same restriction enzyme. Introduce the library into bacteria (each bacterial cell can take up only 1 copy of the vector) Unit 2 study guide: use a labeled fragment of dna complementary to your gene of interest to screen the bacterial colonies.
