BIO 124 Lecture Notes - Lecture 9: Carbon Cycle, Nitrogenase, Antimicrobial Resistance
21 Mar 2016
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Document Summary
Prokaryote genetics: conjugation movement of plasmid dna from one bacterium to another. Major factor in antibiotic resistance: different types of plasmids. Contains the genes which allow a bacterium to produce a pillus used in conjugaction. An r-plasmid is any plasmid that contains a gene conferring antibiotic resistance to the bacterium it is transformed into. R = resistance: transduction dna transfer mediated by a virus. Can be generalized transduction or specialized transduction: transformation uptake of dna directly from the environment. First observed by griffiths in 1928 using. Can help to identify antibiotic resistance gene. Electroporation an electric pulse in introduced so that dna from the environment can be taken up. Heat shock hot then cold: hot -> release of lipids and formation of pores on cell surface. Lowering of membrane potential, which facilitates dna to cross the inner membrane: cold -> raises membrane potential to its original value.
