BIO 209 Lecture Notes - Lecture 12: Five-Prime Cap, Start Codon, World Federation Of Trade Unions

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amethystlouse762

13 Nov 2016

School

Drexel University

Department

Bioscience & Biotechnology

Course

BIO 209

Professor

Loudon Robert P.

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Document Summary

Initiator amino acid is methionine: not formylated. Initiator complex forms at the 5" end of mrna: not a specific sequence like in prokaryotes. Prokaryotes have shine- dalgarno sequence that positions the small ribosomal subunit to allow alignment with the start codon: details: 7-mg cap on 5" end of mrna is bound by cap-binding proteins (cbp) Cbp helps with recognition and binding of the small ribosomal unit. Complex moves 5" to 3" on mrna until it finds the start codon: once found, initiation factors fall off. Chain elongation: aminoacyl-trna binds to a site. Binds to factor-binding center when the correct codon-anticodon interaction occurs: hydrolyses bound energy is released. P-site trna moves to e-site: a-site trna moves to p-site, mrna moves by three nucleotides. This allows the next codon to be exposed: hydrolyzes gtp to facilitate translocation. Peptide bond forms: peptidyl transferase- cataltic activity that is specific to rna, utalyzes activity of gtp hydrolysis.

Related Questions

Put the following list of events in the correct order following the steps required for eukaryotic gene expression: i. The ribosome shifts by one codon ii. A methionine-charged tRNA binds to the first AUG codon from the 5â end of the mRNA iii. An amino acid charged tRNA with the correct matching anticodon binds to the A-site iv. The amino acid from the tRNA in the P-site binds to the amino acid of the tRNA in the A-site v. The large ribosomal subunit binds vi. The small ribosomal subunit binds to a recognition sequence on the mRNA

violetbeetle442

Please please please I reallly need help with the following 5 questions.

Question 1

Which of the following is responsible for directing the prokaryotic ribosome to the +1 start site:

	a.	the 3' UTR
	b.	the AUG codon
	c.	Kozak sequence
	d.	Okasaki fragment
	e.	Shine Dalgarno sequence

Question 2

In translation, mRNAs are READ in the:

	a.	5' to 3' direction and polypeptide chains are synthesized amino to the carboxyl ends
	b.	5' to 3' direction and polypeptide chains are synthesized carboxyl to the amino ends
	c.	3' to 5' direction and polypeptide chains are synthesized carboxyl to the amino ends
	d.	3' to 5' direction and polypeptide chains are synthesized amino to the carboxyl ends

Question 3

Which of the following terms describes a tRNA to which an amino acid has been added:

	a.	charged tRNA
	b.	activated tRNA
	c.	stimulated tRNA
	d.	hydrolyzed tRNA

Question 4

The ability of some tRNA to recognize more than one codon in mRNA is explained by which of the following:

	a.	a reading frameshift
	b.	a reading mistake
	c.	Redundancy Theory
	d.	Wobble Hypothesis

Question 5

One essential component in eukaryotic mRNAs recognized by translation initiation factors is the

	a.	TATA box
	b.	AUG initiation codon
	c.	7-methylguanosine cap
	d.	Shine-Dalgarno sequence

blackporcupine6

Match the following:

1	Region which lies upstream of thetranslation start site contains the Shine Dalgarno sequence
2	Sequence added to the 3' end of thepre-mRNA which affects mRNA stability
3	Establishes the reading frame andspecifies the first amino acid of the protein chain
4	DNA sequence that the transcriptionapparatus recognizes and binds to initiate transcription
5	Expressed sequences of DNA that arenot removed during alternative splicing
6	Signals the end of translation andthe protein molecule
7	Group of three nucleotides that codefor an amino acid
8	Site begins the coding region of thegene and is located 25-30 nucleotides downstream of the TATAbox
9	Non-coding sequences of DNA thatintervene within coding regions of a gene
	10 Ribosome binding site ineukaryotes; functions in the initiation of translation and mRNAstability
11	A sequence which lies downstream ofthe coding region of the gene; determines the location of the 3'cleavage site in the pre-mRNA molecule
12	Sequence of nucleotides at the 3' endof the mRNA that is not translated into protein; affects thetranslation and stability of the mRNA molecule

A.	5' untranslated region
B.	AAUAAA consensus sequence
C.	Promoter
D.	Transcription start site
E.	3' untranslated region
F.	Introns
G.	Initiation codon (AUG)
H.	Poly(A) tail
I.	5' cap
J.	Termination codon (UAA, UGA, UAG)
K.	Exons
L.	Sense codon

ivorymacaw91

BIO 209 Lecture Notes - Lecture 12: Five-Prime Cap, Start Codon, World Federation Of Trade Unions

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