BIOL 141 Lecture Notes - Lecture 2: Serial Dilution, Asepsis, Pipette
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This is a 100-fold dilution: then take 10 microliters from tube a and put in tube b, add 990 microliters of water. This is another 100-fold dilution: tube b will be a 10,000-fold dilution. Model organism: when target organism/system (e. g. human) is not practical or ethical to use in experiment, a model organism is used instead. Ethics considerations: when vertebrates are involved, scientists must have work approved by. Experiments should use the lowest possible animals (e. g. bacteria rather than mice) and the fewest animals possible. They should be treated as humanely as possible. Common model organisms: e. coli bacteria, s. cerevisiae yeast, c. elegans nematodes, Labeling of samples: standard labels should include your name (or group name), lab section date, material in the tube, concentration (if applicable), and any treatments. Include table of contents with experiment date, title, and page number.
