BIOL 142 Lecture Notes - Lecture 20: Dna Microarray, Dna Profiling, Tandem Repeat
30 Apr 2018
School
Department
Course
Professor
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Document Summary
Describe the non-coding, repetitive dna sequences found in eukaryotic genomes called short tandem repeat (str) sequences. Explain how minisatellites, or variable number terminal repeats (vntrs), are used for dna fingerprinting. Describe how gene duplication can give rise to gene families and how the alternative-splicing hypothesis might account for the small number of genes in the human genome. Use a dna microarray to identify differences in gene expression in cells subjected to different conditions: many eukaryotic genomes are dominated by non-coding, repetitive dna sequences that occur between genes or inside introns. In addition, eukaryotic genomes are much larger than bacterial and archaeal genomes, also presenting a challenge for sequencing and analysis. Protein-coding sequences constitute a very small percentage of the human genome, and repetitive sequences make up more than 50 percent. In contrast, over 90 percent of the prokaryotic genome consists of genes. Short tandem repeats (strs): eukaryotic genomes have several thousand loci.
