MCB 3020L Lecture Notes - Lecture 17: Sense Strand, Coding Strand, Enzyme
Document Summary
Replication- duplication of dna prior to cell division. Biotech and replication: error prone pcr, mutator strains- e coli. 3)muts ( mismatch repair) (difficient in 3" to 5": insertion of novel bases. Nucleic acids separate according to size by electrophoresis in an arg rosegel. Detection bands indicate how far the dna traveled. What is the central dogma: dna (transcription, rna (translation, protien. Rna differs in dna in having the ribose as the sugar and the base is uracil instead of thymine. Rna is usually single stranded ss = single stranded ds= double stranded mrna mrna ( messanger rna)- Class of rna molecules that dna uses as messengers to the rest of the cell. Transcription- process in which information from dna is converted to rna. Sense strand: stand of dna equivalent in sequence to mrna. mrna contains information for one ore few genes. Identical in sequence to the coding strand except it contains u instead of t.
