MCB 3020L Lecture Notes - Lecture 27: Gene Gun, Microinjection, Ethylene Glycol
Document Summary
Different kind of vecotr that can accept larger peices of foreign dna than a plasmid can. Technique by which small samples of dna can be quickly amplified, that is increased to quanitties large enough fora nalysis. Incubator that can be set for the desired temperature, times and numbers of ccles. Made possible by isolation of a thermophilic bacteria"s dna polymerase (thermus aquaticus) Newly made dna is tagged with a flourescent dye so that the level of flourescence can be mesaured after every pcr cycle. Reverse transcription pcr use viral rna or a cell"s mrna template and the enzyme reverse trascriptase makes dna from the rna template the dna is then amplified. Can only be used to amplify relatively small sequences of dna. Steps of protoplast fusion: bacterial cell walls are enzymatically digested, producing protopalsts, in solution proplasts are treated with ethylene glycol, protoplasts fuse, segments of the two chromosomes recombine, recombinant cell grows new cell wall.
