PCB 4594 Lecture Notes - Lecture 4: House Mouse, Wound Healing, Chromatin
Advantages of Mus musculus.
MOUSE.
-Mammals.
-Well characterized genome and biology.
-Extensive genetic tools and techniques.
-Many mutants and strains already created
Four levels that gene expression can be regulated
1. Transcription
2. Post Transcription
3. Translation
4. Post translation
Transcription factors
Can bind to DNA to either block or stimulate transcription
How do regulatory proteins gain access to the bases of DNA?
Major grooves
What do regulatory proteins possess?
DNA-binding motifs
General TF
Required for transcription initiation & for proper binding of RNA polymerase to DNA
Specific TF
Regulate transcription in certain cells or in response to signals.
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Document Summary
Four levels that gene expression can be regulated: transcription, post transcription, translation, post translation. Can bind to dna to either block or stimulate transcription. Required for transcription initiation & for proper binding of rna polymerase to dna. Regulate transcription in certain cells or in response to signals. Regulate temporal and spatial expression of the gene. Genes can only have one enhancer that responds to different tf. Genes may have multiple enhances that respond to different tf. Produce proteins which are tf and contain homeodomains. Bind directly to dna and allow them to influence transcription. Makes it easier for tf and rna polymerase to access the dna. Dicer chops double stranded rna into small pieces of rna. Recognizes different splice sites in different tissue types. Proteins to be degraded are tagged with this. Local signaling in animals. growth factors stimulate nearby target cells to grow and divide.
