PCB 3063 Lecture Notes - Lecture 11: Dna Ligase, Dna Replication, Exonuclease
Document Summary
Back up and remove the incorrect nucleotides. 5" --> 3" exonuclease: remove the primers laid down by primase and replace them with dna nucleotides. Dna ligase catalyzes the formation of a phsophodiester bond without adding another nucleotide to the strand. After the last nucletide of the rna primer has been replaced, there still remains a? nick. Tus in e. coli termination protein; blocks the movement of helicase, thus stalling the replication fork and preventing further replication. Dna polymerase alpha initiation of nuclear dna synthesis and dna repair; has primase activity. Lagging strand synthesis of nuclear dna, dna repair, and translesion dna synthesis. Which of the following is true regarding the melting temperature (tm) of dsdna? the tm increases with g + c content. 20 (g = 3 , c = 3, a = 2, t = 2) In vertebrates, dna replication is initiated at multiple sites within linear chromosomes bound by a licensing factor.
