PCB 3063 Lecture Notes - Lecture 10: Chromatin Remodeling, Nucleosome, Chromatin
Where do activators and repressors interact with general transcription factors
At the promoter
Gene expression can be controlled through what process?
RNA silencing
Chromosome territories
portions of the nucleus that certain chromosomes or parts of chromosomes are attracted to
Interchromosomal
domains
Channels between chromosomes are called
Transcription factories
Nuclear sites that contain most of the active RNA polymerase and transcription regulatory molecules
Chromatin remodeling
-A form of decondensing DNA
Chromatin can be remodeled in 3 diff ways
*1. Change a nucleosome by altering components, changing a histone out for a diff one
2. Reposition/remove a nucleosome on the gene region
3. Change the DNA itself through covalent modifications*
Do we need chromatin remodeling?
Yes! It MUST occur to allow DNA to be accessed by DNA binding proteins
Why are histone tails important for histone modification
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Document Summary
Where do activators and repressors interact with general transcription factors. Chromosome territories portions of the nucleus that certain chromosomes or parts of chromosomes are attracted to. Nuclear sites that contain most of the active rna polymerase and transcription regulatory molecules. Chromatin can be remodeled in 3 diff ways. Change a nucleosome by altering components, changing a histone out for a diff one: reposition/remove a nucleosome on the gene region, change the dna itself through covalent modifications* It must occur to allow dna to be accessed by dna binding proteins. Why are histone tails important for histone modification. Because the tail has most of the positive charge, you can change it by: Adding negative charges =a neutral tail which affects the way the histone interacts with the dna. Also by methylation: (when you methylate dna it shuts it down. ), but when you meth. a histone it makes them slippery and allows them to slide around and get translated easier.
