MCB 3020C Lecture Notes - Lecture 16: Metagenomics, Transcriptomics Technologies, Complementary Dna
Document Summary
Entire complement of rna (transcriptome), proteins (translatome), or metabolites (metabolome) produced under certain conditions. Metagenomics: analyzes pooled dna or rna from environmental sample containing organisms which have not been isolated/identified: metagenome: total gene content of microbial community. Examples of metagenomic studies: several environments, extreme environments have low diversity, so community dna can be assembled into individual genomes, most genes from natural habitats are viral, can analyze for presence/distribution of specific microbial groups. Transcriptomics: global study of transcription, monitors total rna under a growth condition: two main approaches: microarrays and rna-seq. Rna-sequence analysis: all rna converted into cdna and sequenced, which genes are transcribed, how many copies of each rna are made, measures mrna expression. Identifies long untranslated regions: discovers noncoding rnas. Proteomics: genome-wide study of structure, function, and activity of an organism"s proteins. Proteome: all proteins encoded or only those present at a given time (translatome)
