BSC 219 Lecture Notes - Lecture 21: Taq Polymerase, Thermus Aquaticus, Molecular Cloning
Document Summary
Multigenic (polygenic): involve the interaction of dozens of different genes in combination with lifestyle and environmental factors. Caused (cid:271)y (cid:373)utatio(cid:374)s or alleles i(cid:374) ge(cid:374)es that o(cid:374) their ow(cid:374) do(cid:374)"t (cid:272)ause a pro(cid:271)le(cid:373) Mutations in genes have minor effects (gene a) Mutations in some genes have moderate effects (gene b) When all combined (you get the disease phenotype) Simple, rapid and sensitive in vitro technique to make many copies of a molecule of dna. Can start with a simple piece of dna and make billions of copies. Dna template reverse transcribed))- 100 bp to thousands. Can be double or single stranded (genomic dna or cdna (made when a mrna is dntp datp, dttp, dgtp, dctp. Taq dna polymerase (isolated from microbe thermus aquaticus) Proper concentration is crucial- too little = inactive, too much = unstable. Short pie(cid:272)es of dna (si(cid:374)gle stra(cid:374)ded a(cid:374)d (cid:272)o(cid:373)ple(cid:373)e(cid:374)tary to 3" e(cid:374)ds of target. Dna shouldn"t be complementary to its self (end with g or c)
