MICRO 302 Lecture Notes - Lecture 6: Atomic-Force Microscopy, Confocal Microscopy, Endospore
28 Jun 2017
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Studying microbial structure: variations of light microscopy, electron. Diagram the use of a light stop in darkfield microscopy. Light enters the microscope for illumination of the sample while the background remains dark. Compare phase-contrast to dark field and confocal microscopy. - phase contrast is like a 3d model of the organism, dark field illuminates the specimen and keeps the background dark for optimal contrast and confocal microscopy or fluorescence microscopy. Explain how fluorescence microscopy differs from light microscopy and discuss different staining techniques used in fluorescent microscopy. Fluorescence implements a glowing dye that stains portions of the organism where light microscopy stains the entire organism either purple or pink depending on ts structure. Compare the effects of light and electron beams on resolution. - electron beams are obviously a characteristic of electron microscopes while light beams are an effect of the standard light microscope. The resolution of the light microscope is directly related to the wavelength of the light.
