BIOL 150 Lecture Notes - Lecture 8: Rna Polymerase Ii, Palindromic Sequence, Tata Box

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15 Mar 2018
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Biol 150 principles of molecular & cellular biology. We reviewed mechanism of transcription in bacteria, especially the role of a palindromic sequence forming structures in dna ( hairpins ) to aid termination. I hope the exercise helped you understand this complicated process more clearly. We turned to look at how eukaryotic cells transcribe dna to make mrna. The overall process is quite similar, but eukaryotic transcription has several complications: There are three rna polymerases; mrna is made primarily by rna pol ii. Rna pol ii uses a protein tfiid ( tf2d ) to bring the polymerase to the tata box (promoter). Rna polymerase ii has a tail to binds proteins that alter the mrna while it is being made! The original transcript (the rna transcribed from the dna) is therefore not the same as the final mrna molecule. The proteins altering the mrna make 3 changes to the transcript:

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