MARKET 1 Lecture Notes - Lecture 11: Acetonitrile, High-Performance Liquid Chromatography, Hexane
Document Summary
The frits prevent particles blocking the column. A guard column to protect main column from strongly binding contaminants. Normal columns are commonly 10 30 cm long with id (internal diameter) 2 5 mm, packing size 3 10 m, 50. Micro columns are commonly 3 7. 5 cm long with id 1 . 4. 6 mm, packing size 1. 8 3. 5 m, 100 000 plates/m. Configuration of a hplc system: column: packing materials, columns are packed with small diameter porous particles (3 m, 3. 5. R group can be various different groups, which means that the sp may have different properties depending on this. Configuration of a hplc system: separation modes. + water-miscible organic solvent (methanol, acetonitrile: separation modes: normal phase/ adsorption chromatography, useful for, water-sensitive compounds, geometric isomers, cis-trans isomers, class separations, chiral compounds, column packing is polar (e. g. silica gel) and mobile phase is non-polar (e. g. hexane) The more polar the solvent, the higher the.
