BIOE-257 Lecture 9: BIOE 257 lecture 9
8 Feb 2019
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Document Summary
The tm depends on: the temperature at the middle point of transition (fig. a, content of g-c base pairs (fig. b, size of dna (fig. c, ph. A basic mechanical property quantifying the stiffness of dna. The maximum length to lose continuity of local curvature. Storing and encoding genetic information: physical & chemical functions. Be self-assembled from complementary dna strands through non-covalent bonds. Be connected via enzyme-catalyzed assembly through covalent bonds. Enzymes: link two dna strands (gluing, cleave dna backbone (scissors, recognize and cleave dna (table saws, remove nucleotide bases from dna termini(sanders, selectively degrade ssdna (trimmers) Linear dna applications: dna-based manipulation of nanoparticles and nanotubes, dna-nanoparticle based sensors, dna guided protein assembly, sensors based on dna-protein conjugates, 2d and 3d assembly of nanoparticles. Scanning force microscopy: commercial sfm tips can have stiffness low as ~10mn/m; can measure forces as low as 10pn, etched optical fibre/glass microneedles are ultra-soft, ~1. 7 mn/m; force precision of ~1 pn.
