BISC 130 Lecture Notes - Lecture 9: Lac Operon, Lac Repressor, Operon

31 views2 pages
Chapter 16: Gene Expression
I. controlling expression of genes
II. Genes (DNA) mRNA proteins affect cellular activities by
acting as signaling molecules, causing chemical reactions, etc.
a. Not all genes need to be made into proteins/expressed at all
times
i. Expressed- “on”, not expressed- “off”
III. Multicellular organisms: different types of cells express different
genes for different functions
IV. Multicellular and unicellular organisms: need to respond to their
environment; often related to the available resources
V. Most control of gene expression is at the transcriptional stage
a. Prevent/encourage transcription
i. DNA mRNA
b. Proteins very frequently used by the cell to control gene
expression
i. Bind to DNA in a sequence specific manner
VI. Transcription factors: proteins that bind DNA and affect
transcription levels of a gene or genes
VII. The lac operon: bacterial gene regulation at the transcriptional
level
a. Operon- a set of genes transcribed together
i. Genes are related and controlled by the same promoter
so that the genes are transcribed together into a single
strange of mRNA (happens only in bacteria)
VIII. Lac operon- contains several genes that encode proteins that
break down lactose; controlled on two levels
a. Lactose: a disaccharide that must be broken in two before
being used by the cell as energy by the cell
IX. Situation 1: Cells only want to express genes for lac operon if
lactose is available to be broken down; wasteful to make useless
proteins
a. uses a protein called lac repressor protein
b. if lactose is not present: lac repressor binds to DNA operator
region of lac operon, downstream of the promoter
i. sequence specific
ii. physically blocks RNA polymerase from transcribing
iii. expression of the lac operon is repressed—“off
iv. repressor- transcription factor that turns “offgene
expression
c. If lactose is present: lactose sugar molecule binds to lac
repressor protein, changes the shape so that it cannot bind to
DNA and stop the RNA polymerase from transcribing the lac
operon
find more resources at oneclass.com
find more resources at oneclass.com
Unlock document

This preview shows half of the first page of the document.
Unlock all 2 pages and 3 million more documents.

Already have an account? Log in

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

Related Questions