7.012 Lecture Notes - Lecture 1: Silent Mutation, Wild Type, Plasmid
Document Summary
Example: imagine a mutant yeast, that unlike all other yeast, cannot synthesize arginine. To nd the gene, we make a plasmid library, in which each plasmid has a wild type copy of a different gene. We then transform a different mutant yeast colony with a different plasmid. We then see which one rescues, turn the mutant into a wild type, the mutant phenotype. The conclusion we can draw from this is that the plasmid transformed in that rescued colonies contains the gene of interest. You must be working with recessive mutations (usually a loss of function) Adding wild type to a gain of function will not give us a rescued phenotype. Must be in something that you can transform genetically. Snps single nucleotide polymorphisms, meaning we are looking at a single base pair difference at a given position among a population.
