7.012 Lecture Notes - Lecture 1: Rna-Dependent Rna Polymerase, Dna Virus, Dna Replication

• School: MIT
• Department: Biology
• Course: 7.012
• Professor: Eric Lander

Document Summary

Purines: (a and g) is a 6 membered and 5 membered ring. Pyrimidines: (t, c) is a 6 membered ring. To make a chain of nucleotides, we polymerize them by a dehydration synthesis. The phosphate hanging off of the 5" carbon connects to the 3" carbon. Semi conservative replication: replicated dna is made up of one new strand an one old strand. Old dna contains n-15 isotope and the new dna contains n-14 isotope. Time zero: one dna molecule where both strands are n-15. Time one: two dna molecules where both have one strand of n-15 and one strand of n-14. Time two: four dna molecules where two have one strand of n-15 and one strand of n-14 and two have both strands of n-14. Primer: a piece of dna that matches the template that can use the oh at the 3" carbon end to grab the next nucleotide to extend the strand.