BIOS10115 Lecture Notes - Lecture 4: Electron Microscope, Microscopy, Ribosomal Rna
3 Dec 2016
School
Department
Course
Professor
Document Summary
Phase contrast: difference in contrast, live samples, requires phase plate ring. Fluorescence 0 the ability to emit light (of a different wavelength) when exposed to light: natural, dyes, tags , some techniques allow cells to be alive, improves contrast and detection. Resolution 0. 2 nm (1000x better than lm) Transmission em (tem: through , internal structures. Em: many types they all use some form of light, resolution 0. 2 um (micrometer, improving contrast improves detection, live/dead cells, resolution 0. 2 nm (nanometer, tem vs sem, dead cells. Organisms must have a name: common names become confusing. Two kindgoms plants and animals (when bacteiera discovered, they were considered plants) Binomial (two) nomenclature (name: genus, specific epithet (description) Species: escherichia coli (e. coli, homo sapiens (h. sapiens) Plants and animals: interbreed and produce fertile offspring. Microbes: 70% of dna identical or by mutual consent of scientists. Often change when new info is obtained. 2 kingdom system (pre-1860: plant, animals. 3 kingdom 1869: plant, animal, protista.
