BIOS10162 Lecture Notes - Lecture 1: Trp Operon, Lac Operon, Tata Box
31 Aug 2016
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Document Summary
Polycistronic: multiple promotors, 1 mrna multiple proteins. Individual exons code for 1 mrna single protein// polypeptide. Transcription factors required- bind areas in the promotor. Tfiid binding- rna polymerase ii and transcription factors bind complex. Different gene transcription regulated by specific transcription factors. Regulator protein binds to regulator region joins complex. Transcription regulated by regulation of general//specific transcription factors. Epigenetics: study of heritable changes in gene expression w/o involving change in. Exposure to environment changes dna gene and protein expression. Promotor regions have islands of c"s and g"s= cpg (c phosphate g) ions. Normally un-methylated dna coiling around histones loose allows transcription. C"s can be methylated (+methyl group) down regulation of transcription without changing the gene. Investigated bpa (environmentally hazardous) put in mothers" diets. Looked for methylation: found a gradient of cpg methylation -- (brown had lots of methylation) De-methylation of parent cells but gametes can be re-methylated; random (*bpa affected methylation in offspring not the mother)
