BIOL 2301 Lecture Notes - Lecture 11: Tata Box, Repressor, Transcription Factor

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indigooyster587

15 Aug 2018

School

Northeastern University

Department

Biology

Course

BIOL 2301

Professor

Christopher Richardson

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Document Summary

Housekeeping genes key genes that are constitutively expressed at lowlevels or sometimes beyond basal levels. All genes when off make some residual amount af protein in eu . when. Many processes are common to all cells any two cells in a single: some r act by binding to enhancer sites. : block activators from binding to enhancer sequences. Some r bind to silencers to slow transcription. Rna poly access to promoter a. eliminate almost all transcription i " Most r act as inhibitors : bind to activators directly iii. iv. a. b. inactivate the activators bk the inhibitors stop the activators from binding to the enhancers positive regulation , repressible i . ii. iii. Many transcriptional activators can be categorized by characteristic dna binding protein folds. Helixmotif has pairs of helices separated by a linear chain of amino acids. Zinc motif organism: transcriptional have many proteins.

Related Questions

PLEASE HELP ANSWER ALL PARTS A-E

âExon 11 of the insulin receptor gene (INSR) is alternatively spliced in a developmentally and tissue-specific manner. Linker scanning mutations in a 5' GA-rich enhancer in intron 10 identified AGGGA sequences that are important for enhancer function. Using RNA-affinity purification and mass spectrometry, we identified hnRNP F and hnRNP A1 binding to these AGGGA sites and also to similar motifs at the 3' end of the intron. The hnRNPs have opposite functional effects with hnRNP F promoting and hnRNP A1 inhibiting exon 11 inclusion, and deletion of the GA-rich elements eliminates both effects. We also observed specific binding of hnRNP A1 to the 5' splice site of intron 11. The SR protein SRSF1 (SF2/ASF) co-purified on the GA-rich enhancer and, interestingly, also competes with hnRNP A1 for binding to the splice site. A point mutation - 3UâC decreases hnRNP A1 binding, increases SRSF1 binding and renders the exon constitutive. Lastly, our data point to a functional interaction between hnRNP F and SRSF1 as a mutant that eliminates SRSF1 binding to exon 11, or a SRSF1 knockdown, which prevents the stimulatory effect of hnRNP F over expression.â PlosOne 6: e27869.

A. What is the function of a SR protein?

B. What is the function of a hnRNP?

C. What is the significance of having two IR isoforms?

D. What is the significance of the G-rich elements in intron 10?

E. How does genetic disruption of the SRSF1 binding site on exon 11 effect INSR splicing?

In Eukaryotic transcription, what is the difference between an enhancer, a mediator, a coactivator and a trascriptional activator protein? In your answer, for each one include (a) if it's the term refers to a DNA sequence or a protein, and if it's a protein (b) if it interacts with DNA, with another protein, or with both. Also how are enhancers different from promoters in affecting gene transcription in eukaryotes? Make sure that you include in your discussion: (c) their relative position to the transcription initiation site; (d) the role of binding sites for proteins; (e) how each affects initiation and transcriptional regulation (in the latter, make a distinction between the core and the regulatory promoter

lilacseal677

What role do insulator sequences play in the regulation of eukaryotic transcription?

A		They block communication between enhancers and nontargeted promoters
B		They block binding of transcription factors to cis-acting promoter elements
C		They block binding of transcription repressors to cis-acting promoter elements
D		They block binding of transcription factors to enhancers
E		They block binding of transcription factors to silencers

What would be the effect of a mutation in the lacI gene that prevented the repressor from binding to lactose?

A		The lac Z, Y, and A genes would not be expressed.
B		The lac Z, Y, and A genes would be expressed constitutively
C		The lac Z, Y, and A genes would be repressed by lactose.
D		The lac Z, Y, and A genes would be induced by lactose.
E		The lac Z and Y genes would be repressed but not the lac A gene.

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BIOL 2301 Lecture Notes - Lecture 11: Tata Box, Repressor, Transcription Factor

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