BIOL 2301 Lecture Notes - Lecture 22: Lac Operon, Transgene, Bamhi
15 Sep 2016
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Piece of dna moved from one background to another (usually different organisms) We now have other techniques, not just recombinant. Take dna fragment, put in plasmid, put in organism where it is replicated. By moving gene, can make these drugs. Transgene: gene of one organism expressed in another. Rnai: put plasmid that makes dsrna for you. Insulin: what tells cells to take up sugar from blood: given as treatment for diabetes. Cells that make insulin are destroyed by immune system. No signal that there is insulin in body. Tells cells to take in glucose from blood stream. Two cys- link to form sulfur-sulfur bond: can help proteins have structure, send signal. The gene that encodes human insulin has 3 introns and 2 exons: Mrna dna gets us just the coding part (which is what bacteria deal with) reverse transcriptase using pcr make many copies of cdna. How to get the gene you want from cdna.
