BIOLOGY 1101 Lecture Notes - Lecture 6: Gel Electrophoresis, Dna Paternity Testing, Hybridization Probe

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Dna technology is any technology that allows us to analyze and/or manipulate dna. There are many uses of biotechnology, but many use the same small number of tools, just in different orders or at different times. Chop up dna from a donor species that exhibits a trait of interest. Amplify small samples of dna into more useful quantities. Insert pieces of dna into bacterial cells or viruses. Identify colonies of bacteria or viruses that have dna for a trait of interest. Restriction enzymes: enzymes that cut dna at specific sequences. In nature: a defense mechanism by bacteria against viral infection (some viruses infect bacteria) Some restriction enzymes create sticky ends that can be resealed; important for adding in new sequences. Pcr (polymerase chain reaction): a powerful tool that allows researchers to quickly produce many copies of a sequence. Pcr uses dna polymerase (similar function, but copies dna)

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