Recombinant DNA Technology
The questions all relate to the same topic and somewhat interrelate
1. Plasmids used in vitro to clone foreign DNA fragments are called ___
A. Transgenic.
B. Clones.
C. Vectors.
D. Conjugants.
2. VNTR alleles are hypervariable regions of human DNA that differ from each other in:
A. Location of internal sites recognized by restriction enzymes.
B. Variable number of point mutations.
C. Number of copies of an internally repeated DNA sequence.
D. Variable location on different chromosomes.
3. Probability calculations are used in forensic applications of DNA fingerprinting to determine if:
A. DNA from two different sources has matching alleles.
B. DNA samples were degraded before analysis.
C. A match between alleles of different DNA samples might have occurred by chance.
D. An unknown DNA sample is from a male or a female.
4. Plasmid vectors used in cloning often contain a gene for the N-terminal 146 amino acids of the enzyme β-galactosidase. What is the purpose of including this gene in the vector?
A. It allows selection of E. coli host cells that contain the plasmid.
B. It allows selection of E. coli host cells that contain plasmid in which the insert has been ligated.
C. It cleaves the insert to allow it to be ligated into the vector.
D. It enables the plasmid vector to replicate in E. coli host cells.
5.The first gene therapy clinical trial took place in 1990, on patients with SCID (severe combined immunodeficiency syndrome). Is this true or false?
A. True
B. False
Recombinant DNA Technology
The questions all relate to the same topic and somewhat interrelate
1. Plasmids used in vitro to clone foreign DNA fragments are called ___
A. Transgenic.
B. Clones.
C. Vectors.
D. Conjugants.
2. VNTR alleles are hypervariable regions of human DNA that differ from each other in:
A. Location of internal sites recognized by restriction enzymes.
B. Variable number of point mutations.
C. Number of copies of an internally repeated DNA sequence.
D. Variable location on different chromosomes.
3. Probability calculations are used in forensic applications of DNA fingerprinting to determine if:
A. DNA from two different sources has matching alleles.
B. DNA samples were degraded before analysis.
C. A match between alleles of different DNA samples might have occurred by chance.
D. An unknown DNA sample is from a male or a female.
4. Plasmid vectors used in cloning often contain a gene for the N-terminal 146 amino acids of the enzyme β-galactosidase. What is the purpose of including this gene in the vector?
A. It allows selection of E. coli host cells that contain the plasmid.
B. It allows selection of E. coli host cells that contain plasmid in which the insert has been ligated.
C. It cleaves the insert to allow it to be ligated into the vector.
D. It enables the plasmid vector to replicate in E. coli host cells.
5.The first gene therapy clinical trial took place in 1990, on patients with SCID (severe combined immunodeficiency syndrome). Is this true or false?
A. True
B. False
Related textbook solutions
Related questions
Which of the following did Erwin Chargaff observe?
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The melting temperature (Tm) of a DNA duplex is:
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Given your knowledge and the descriptions of each of thesemethods, which of the following does NOT rely on the ability ofnucleic acids to hybridize with each other?
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Which of the following is a possible reason why DNA uses thymineinstead of uracil?
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Which of the following is an enzyme used in cloning to breakcovalent bonds?
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Which of the following could you use to remove the 5' phosphatesafter cleavage of a plasmid with a single type of restrictionenzyme to ensure that the plasmid would not simply fuse backtogether upon addition of ligase?
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Which of the following would you use to create a cDNA librarythat you would not use for a genomic library?
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A plasmid vector typically has which of the followingfeatures?
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Which of the following is FALSE of genomic or cDNAlibraries?
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Which of the following is NOT a typical component of apolymerase chain reaction (PCR)?
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How can a fusion protein be formed?
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Why is green fluorescent protein (GFP) so useful in visualizingfusion proteins in eukaryotes?
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You wish to determine the cellular location of a protein butunfortunately it is not particularly antigenic (can t develop astrong antibody response). How could you solve this problem?
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A positive result for the yeast two-hybrid analysis means thefollowing:
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Which of the following is the approximate size of the humangenome?
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Which of the following is correct about the structure orlocation of genes?
Question 22 options:
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