BIOS 2210 Lecture Notes - Lecture 3: Micrographia, Microscopy, Optical Microscope
9 Sep 2018
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Organisms in the size range 1nm to 1cm. Micrographia (1600s): book of hooke"s drawings to record what he saw under microscopes. Today we have cameras attached to microscopes and color-enhancing compute. At least 100x to see in detail, need to use electron microscope. Parfocal (able to rotate the magnification without changing the plane where the specimen is being observed) omputers here the. Total magnification = magnification of eyepiece x magnification of objective lens. One color of dye, when observing it allows for you to see size/shape. Differential stain that distinguishes two characteristic within the cells. A way to visualize and differentiate cell wall structure. Gram + : on the outside of bacterial cell, on the cell wall, stain purple, l of protein. Gram - : stains pink, membrane is a little protein and another membrane. Important in medicine ctive n purple, lots brane. Creates a dark background and allows you to see through the cells.
