MICR 2123 Lecture Notes - Lecture 5: Dna Replication, Semiconservative Replication, Microbiology
8 Sep 2016
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Document Summary
When the cell divides, dna is replicated so each daughter cell gets a copy of the parental. Replication of cellular dna is semiconservative: each daughter cell receives one parental and one newly synthesized strand. Starts at the origin of replication (ori: elongation- Two replication forks that move in opposite directions around the chromosome: termination- At defined termination (ter) sites located opposite to the origin. Important things to know: the main replication enzyme is the dna polymerase. Adds nucleotides to make a dna daughter strand using the information in the template mother strand. Can only work in direction 5" to 3": after initiation, each replication fork contains two strands: A leading strand, that is the 3" to 5" strand and is replicated continuously in the 5" to 3" direction. A lagging strand, that is the 5" to 3" strand which is replicated discontinuously in stages, each producing an okazaki fragment.
