BIOL 110 Lecture Notes - Lecture 1: Ethidium Bromide, Agarose Gel Electrophoresis, Antimicrobial Resistance
Document Summary
The materials used for the lab included plates with gentamicin and without, microtubes, sterile glass beads, the genetically engineered e. coli bacteria, and an incubator were used. Pcr aspect required the use of dna primers, three distinctive control plasmids, and the machine that performed the pcr. In the final phase, dna ladder, agarose gel, buffer, ethidium bromide, loading dye, a gel electrophoresis rig, and a uv light with a hood were utilized. Throughout the experiment pipettes and disposable tips were made use of. Throughout the experiment, sterile technique was consistently used to prevent contamination of the sample. Gloves were worn at all times and a new pipette tip was used for each solution and substance. The amount of time that containers were open was minimized and the plate lids were only opened slightly rather than taken all the way off. Additionally, everything was properly sterilized and sealed before and during the experiment.
