BIOSC 0150 Lecture Notes - Lecture 10: Optical Microscope, Bright-Field Microscopy, Electron Microscope
9 Feb 2017
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We need a microscope to see most cells and seeing cells helps us make observations: allows us to make observations about cells, the resolution of our eyes 200 microns, two types of microscope. Bright field light microscopy uses light to illuminate small objects: compound microscope. Put thin layer of specimen on a slide: stereoscope/dissecting scope. Can put a whole specimen on it. Magnifying is a bit lower: uses light & lenses, alive specimen. Very little light contrast: dead specimen. Protein: different sites is where an antibody would bind to it"s destination. Epifluorescent and confocal microscope allow you to visualize fluorescent cells: epifluorescent. Like a compound microscope but is adapted to collect light that is reflected from fluorescent antibodies: confocal. Good for looking at tissue that has a lot of depth. Confocal microscopes enable reconstruction of thick specimens by collecting images at different depths: epifluorescent = blurrier.
