BCHM 10000 Lecture Notes - Lecture 7: Hydroxylation, Expasy, Blast

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Slide 1
Nucleic acids
RNAs in the cell belong to several different classes
Messenger RNA (mRNA)
5 to 10% of cellular RNA
carries the genetic information for the synthesis of proteins
Transfer RNA (tRNA)
10 to 15% of cellular RNA
decodes mRNA during protein synthesis
Ribosomal RNA (rRNA)
75 to 80% of cellular RNA
major structural feature of ribosomes
complexed with proteins
BCHM 100 Weeks 3 - 4
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Slide 2
Translation, mutations and the genetic code
in mRNA (and DNA), 1 amino acid is specified by a group of three
bases called a codon
the genetic code is redundant (degenerate)
most amino acids have more than one codon
BCHM 100 Weeks 3 - 4
At 3 codons, it gives you
enough room for error during
mutations (bifurcations).
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Slide 3
Met His Pro Ile
Met Gln Val Cys Ile Pro Ser
Translation, mutations and the genetic code
the first codon that is translated is always AUG (ATG in DNA)
the initiation codon codes for methionine
AUG is used for internal methionine residues also
the first AUG codon establishes the correct reading frame
5'-AGCTCATGCAAGTATGCATCCCATCA-3’
3'-TCGAGTACGTTCATACGTAGGGTAGT-5'
5'-AGCUCAUGCAAGUAUGCAUCCCAUCA-3'
5'-AGCUC-AUG-CAA-GUA-UGC-AUC-CCA-UCA-3'
--- --- --- --- --- --- ---
5'-AGCUCAUGCAAGU-AUG-CAU-CCC-AUC-A-3'
--- --- --- --- ---
ribosome must initiate translation at the correct AUG
sequence starting with AUG (ATG) and ending with a stop codon is an
open reading frame (ORF)
BCHM 100 Weeks 3 - 4
Met-Gln-Val-Cys-Ile-Pro-Ser
Met-His-Pro-Ile
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Document Summary

Nucleic acids: rnas in the cell belong to several different classes. Messenger rna (mrna: 5 to 10% of cellular rna, carries the genetic information for the synthesis of proteins. Transfer rna (trna: 10 to 15% of cellular rna, decodes mrna during protein synthesis. Ribosomal rna (rrna: 75 to 80% of cellular rna, major structural feature of ribosomes, complexed with proteins. Translation, mutations and the genetic code in mrna (and dna), 1 amino acid is specified by a group of three bases called a codon the genetic code is redundant (degenerate) Most amino acids have more than one codon mutations (bifurcations). At 3 codons, it gives you enough room for error during. Translation, mutations and the genetic code the first codon that is translated is always aug (atg in dna) Aug is used for internal methionine residues also the first aug codon establishes the correct reading frame.

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