Please help! Biochemistry homework. I totally donât understand how to do any of these. Thanks so much! No other information was given besides what is on the page.
Below you will derive an equation to express the velocity of an enzyine catalyzed reaction in the presence of a jst algebra... competitive inhibitor kinetic scheme for a single substrate enzyme catalyzed reaction in the presence of a competitive inhibitor e Just as in the absence of a competitive inhibitor, the rate (velocity) of product formation with a competitive Inhiblior present is (a) v-ker [ES] Mass balance. In the presence of substrate and competitive inhibitor, the enzyme can be in one of three forms (ill In blanks);: å..[ ]+[]+[ ] Divide. Divide equation (a) by the mass balance equation: Equilibria: ES and El complexes have dissociation constants that can be expressed as (ill in blanks): c)K1'I (d)K,-I Rearrange equation (c) and (d) to solve for ES and El respectively (ill in blanks) (e) (ES I OlEIlI1 Substitute. Rewrite equation () with the terns ES and El substituted with the right hand side of (e) and (0) Factor & simplify,. Now, on the right hand side of equation (g), I have factored out the [EJ terms, so they cancel, o give (h) [S]/K Finally, the numerator and denominator on the right side of equation (h) can be multiplied by K, and factored to give a sin form of the velocity equation: