11:115:301 Lecture Notes - Lecture 16: Oxidative Phosphorylation, Mitochondrial Matrix, Electrochemical Gradient
22 Sep 2019
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Document Summary
Electron transport proton gradient across inner mitochondrial membrane. Mitochondrial inner membrane is like a capacitor membrane potential = Proton motive force is the sum of: Proton motive force used to drive an atp synthase. The respiratory chain + atp synthase are biochemically separate but linked. Atp synthase resides in the inner mitochondrial membrane by proton motive force. 8-14 hydrophobic c subunits span the inner membrane + form a proton channel f0. Can pick up + drop off h+ Deforms the individual a and b subunits. Protons enter the c ring from the intermembrane space + go into mitochondrial matrix c ring rotates. Y subunit rotates when it touches a b subunit changes conformation + activity. Takes 3 protons 1 complete rotation of rotor. It was found that the atp synthase alone converted adp + pi atp (and the reverse: even in the absence of the proton-motive force, but newly synthesized atp was not released from the enzyme.
