11:115:301 Lecture Notes - Lecture 21: Schiff Base, Nitrogenase, Stereospecificity
22 Sep 2019
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Use atp + nitrogenase make nh3. Nh3 used by glutamate dh make glutamate from which all other aa"s and nitrogenous molecules are made. Glutamate dh is responsible for fixing all nh4+ into proteins. Reductive direction responsible for scavenging up free ammonia + making it into amino acids. A-kg + nh4 --gludh--> schiff base + h2o. Schiff base + nad(p)h + h+ --gludh--> glutamate + nad(p)+ Schiff base: product of an amine + carbonyl. Either use nadh/h or nadph/h as electron donor. Glutamate dh is the first step for all aa biosynthesis. Glu will donate its nh4+ to all other aa"s. Glutamate + glutamine can be nh3 donors. Six families of amino acid biosynthetic pathways (bacteria + plants) The carbon skeletons come from the intermediates of cac, glycolysis, ppp. Families in amino acid tend to preserve the number of contiguous carbons . Synthesis of 3- and 5-carbon amino acids.
