01:119:115 Lecture Notes - Lecture 5: Transmission Electron Microscopy, Electron Microscope, Optical Microscope

• School: Rutgers University
• Department: Biological Science
• Course: 01:119:115
• Professor: Dr.Anne Keating

Document Summary

Tools and techniques used to study cell structure: microscope a. i. Sem scanning a. ii. 2. e. ii. 1. study surfaces a. ii. 2. e. ii. 2. specimen coated with thin film of gold a. ii. 2. e. ii. 3. electron beam strikes the surface a. ii. 2. e. ii. 3. a. Takes cells apart and separates major organelles and cell structures from each other b. ii. Supernatant -> pour into another tube -> centrifuged. Results in subset of cell components to settle and form pellet (bottom) b. ii. 6. b. ii. 7. This is done to prepare specific cell components in bulk. Cellular diversity & characteristics: prokaryotes (fig 6. 5) a. i. ~3. 5 billion years ago a. iii. characteristics a. iii. 1. a. iii. 2. a. iii. 3. a. iii. 4. plasma membrane cytoplasm a. iii. 2. a. cytosol semifluid lack nucleus have nucleoid a. iii. 4. a. a. iii. 4. b. not enclosed by membrane genetic material in the form of double stranded a. iii. 5. a. iii. 6. a. iii. 7. Advantageous to cell to maximize plasma membrane surface area (sa) in relation to its volume c. ii. 3. Ex: box each side 2 cm c. ii. 3. a. i. Ex: box each side 1 cm c. ii. 3. b. c. ii. 3. b. i.