01:119:115 Lecture Notes - Lecture 17: Intron, Chromosome, Dna Barcoding
20 Dec 2016
School
Department
Course
Professor
Document Summary
Many involve nucleic acid hybridization base pairing one strand of nucleic acid to a complementary strand. Genetic engineering direct manipulation of genes for practical purposes. There are a lot of dna sequencing techniques. Divided into two main groups: old and next gen . We"re only going to talk about the old way. Still extremely common, often the best option. Dna sequencing: uses complementary base pairing, is automated- sequencing machines, dideoxyribonucleotide chain termination sequencing. Can sequence dna fragments up to ~1000 bp. Chain termination sequencing: deoxyribose (missing both hydroxyl groups) Requirements- target an sequence denatured into single strand, incubated with the following: primers: short dna molecule, designed to base pair with 3 prime end of template strand. 4 dideoxynucleotides, each labeled with different fluorescence (very low frequency) Set of labeled strands of different lengths generated. Why does the chain terminate?- cannot add subsequent. Dna fragments put in gel, migrate based on size. Detector reads color of each tag as they migrate.
