01:119:115 Lecture Notes - Lecture 17: Polyacrylamide Gel Electrophoresis, Chain Termination, Spectrogram

• School: Rutgers University
• Department: Biological Science
• Course: 01:119:115
• Professor: Stern Cardinale

Document Summary

Dideoxy or chain termination sequencing -> 1st automated procedure -> up to. 1000bp sequencing: next-generation sequencing -> most recent one -> does not rely on chain termination - > up to 100s of million bp sequencing. Dna tools and biotechnology chapter 20. 1, 20. 2, and 20. 4 last chapter included. Dna technology -> techniques for sequencing and manipulating dna. Biotechnology -> manipulation of organisms or their components to make useful products. Nucleic acid hybridization -> the base pairing of one strand of nucleic acid to the complementary sequence on another strand. Genetic engineering -> direct manipulation of genes for practical purposes. Use sequencing -> complete genomes -> evolutionary relationships. Use principle of complementary base pairing to determine gene"s complete sequence of nucleotides. Rna -> ribose but dna -> deoxyribose = lacks 2" oh. Dideoxyribose also lacks 3" oh groups (to which next nucleotide would normally link) Dna to be sequenced -> dented into single strand.