01:119:115 Lecture Notes - Lecture 17: Polysaccharide, Intron, Enzyme

• School: Rutgers University
• Department: Biological Science
• Course: 01:119:115
• Professor: Professor Cardinale

Document Summary

A. common techniques: techniques for sequencing and manipulating dna, nucleic acid hybridization- base-pairing of one strand of nucleic acid to a complementary strand, genetic engineering- the direct manipulation of genes. A. uses principle of complementary base pairing: base pairing determines complete or partial sequence of nucleotides, automated (sequencing machine) C. technique: dideoxyribonucleotide (dideoxy) (important: figure 20. 3: chain termination sequencing method, first automated sequencing developed, frederick sanger developed this method, got the nobel prize in 1980, 1. ) Allows us to determine the sequence of dna fragments: one fragment = ~1000 base pairs, 2. ) Sugar: rna: ribose, dna: deoxyribose, it lacks 2" hydroxyl group (-oh), dideoxy, it lacks 2" hydroxyl group and 3" hydroxyl group, 3. ) Polyacrylamid gel: made of a polymer and acts like a sieve, in this gel, dna can move through it, shorter strands move faster, longer strands move slower, detector sense the label (fluroscent tag), shortest -> longest, output, 6. ) Spectrogram: allows us to determine the sequence of dna.